Home
    Scientific Program
    Registration
    Accommodation
    Contact
The 2nd International Congress on
Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue & Organs (CRYO)
Palacio de Congresos, Valencia, Spain, April 7-9, 2011
 
  Workshop 2 Print

 
Workshop No. 2
HANDS-ON WORKSHOP: OOCYTE VITRIFICATION IN
A CLOSED SYSTEM

(Open for a limited number)

Thursday, April 7, 2011, 09:00-18:00

09:00-13:00 THEORETICAL SESSION
Moderators: Ana Cobo, Spain; Masashige Kuwayama, Japan; Amir Arav, Israel
  
09:00-09:05
Introduction
A. Cobo, Spain
  
09:05-09:40
Fundamentals of Cryobiology
S. Leibo, USA
  
09:40-10:15
Different approaches of Vitrification
G. Vajta, Australia
  
10:15-10:45
Coffee Break
  
10:45-11:15
Vitrification practice made perfect
A. Arav, Israel
   
11:15-11:45
The Cryotop method for oocyte and embryo vitrification?
M. Kuwayama, Japan

   
11:45-13:00
Live demonstration of using the Cryotop Method vitrifying oocyte with explanation by
A. Cobo,
Spain and M. Kuwayama, Japan

  
Aim of the theoretical session: To introduce the biophysical and biochemical principles involved in cryobiology:
  

Ice formation

Slow freezing procedures

Seeding

Freezing curves

The physical principles of Vitrification, introduction to minimum volume methods, cooling rates and different Vitrification devices
  
13:00-14:00 Lunch Break
  
14:00-18:00
HANDS-ON WORKSHOP:
OOCYTE VITRIFICATION IN A CLOSED SYSTEM
Directors: Amir Arav, Israel; Yona Barak, Israel
  
We will demonstrate the different techniques of closed system Vitrification.
 
Each participant will try to vitrify oocytes using Sealed SOPS and the VitMaster. We will review and troubleshoot problems which occur during Vitrification, revealing the methods required to solve these problems.
 
1) Solution effect - are we using a safe solution?
2) Technical skills - how to produce a small drop containing oocytes
3) Open vs Closed system, LN vs. Slush vitrification
4) Using homemade cryo-microscope, explore the crystallization, de-vitrification, fracturing and recrystallization phenomenons
 
Practice Makes Perfect
In addition to the mice oocytes that will be used in the workshop, each participant will have 1000 Glass beads of 100um each (size of oocyte) which will be use for practicing the vitrification procedure.
 
      
Workshop Fee: €2000 (Two thousand euro)
 
*Workshop & Congress Fee
: €2400 (Two thousand four hundred euro)
*Grants the participant access to the IVI Congress and CRYO Congress
    
   REGISTRATION IS RUN ON A FIRST COME FIRST SERVE BASIS
      
 IVI CONGRESS
 Scientifc Program:
http://comtecmed.com/ivi/2011/sp.aspx
 Registration: http://comtecmed.com/ivi/2011/Registration.aspx


Home   
Copyright © 2007 Comtecmed.com. All rights reserved.   The website was last updated on 05/01/2011 Created by    WebStudio.co.il