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The 1st International Congress on
Controversies in Cryopreservation of Stem Cells, Reproductive Cells,
Tissue and Organs (CRYO)
Palacio de Congresos, Valencia, Spain, May 27-30, 2010
 
  Topics to be discussed Print
Basic understanding of vitrification
  • Biosafety of vitrification - the issue of contamination using an open system
  • Rationale of cryopreservation by Very High Cooling and Warming Rates
  • Basic principles of vitrification
  • Vitrification of bovine and equine embryos - practical considerations
  • Cryopreservation of oocytes - slow freezing vs. vitrification
  • Clinical application - what are the limits?
What can go wrong in cryopreservation
  • Errors that can occur when applying cryopreservation technology in the real world, and how to minimize these errors
  • What can go wrong during vitrification
  • Cellular damage during cryopreservation
Sperm freezing in primates
  • Cryopreservation of spermatozoon in rhesus and cynomogus monkey
  • Intracellular ice formation in spermatozoa?
  • Freeze dried sperm  
Freezing of embryonic & adult stem cells - various techniques 
  • Cryopreservation of human embryonic stem cells
  • Large animals models in regenerative medicine: The need and the limits
  • Tumorigenicity of pluripotent stem cells
  • Cryopreservation of adult stem cells  
Cord blood banking, biology, transplantation and tissue regeneration 
  • Cord blood banking - public vs private network
  • Cord blood cryobiology: liquid nitrogen, cryoprotectant vs. lyophilization
  • Cord blood transplantation: Do we know the best option for alternative donor transplant: MUD vs. CB vs. Haplo
  • Cord blood transplantations: Strategies to facilitate engraftment, do we know the best one? 
  • Cord blood tissue regeneration potential: Mesenchymal stem cells (MSCs) heart, vascular, neurology. Does it exist at all?  
Stem cell - preservation of future medicine
  • Adult stem cells vs. embryonic stem cells
  • Somatic cells into stem cells. Reprogramming!
  • Preclinical and clinical trials of stem cells therapy
  • Pluripotent stem cells in medical research
  • The ethics of manufacturing gametes in the lab
  • The use of cord blood stem cells for tissue therapeutics
  • Future use of stem cells in medicine
  • Future medicine of pluripotnet cells 
Animal embryo freezing
  • Domestic animal embryo freezing
  • Freeze dried cells for nuclear transfer
  • Animal cloning from frozen dead body
Fertility, tissue and organ preservation
  • What to freeze: whole ovary, cortex or other?
  • Duration of function of fresh and frozen ovary transplants
  • Where to transplant: Heterotopic or orthotopic sites?
Embryo Freezing
  • What is the best stage for freezing? From @PN to blastocyst
  • Neonatal outcome from the vitrified cleavage stage to embryos
  • Vitrification of blastocycsts: it is the way to go!
  • Embryo freezing: A key to elective single embryo transfer (eSET)
Core Dynamics - Freeze dry of stem cells
 
Cryo-Bank
  • Medical and social aspects of cryopreservation of oocytes for fertility preservation
  • Legislation of cryopreservation of gametes, tissue and regulation of transportation between countries
  • The use of cryopreservation of tumor tissue for preparation of tumor cell vaccines and for future evaluation of molecular pathways 
Is it possible to restore sperm from cryopreserved tissue or should we always get a fresh one?
  • Advantages of using cryopreserved TESE against freshly obtained tissue
  • Multipotent adult germ line stem cells. New therapeutic hope? 
  • Clinical evaluation of the harmful effects of sperm freezing and its current indications  
Workshops
  • Oocyte and embryo vitrification - The cryotop method
  • Vitrification in a closed system
  • Basic knowledge in cryopreservation and vitrification of oocytes and embryos
  • How to manage and run a cryopreserved oocyte bank
  • How to establish a public CBB and how to get ready for fact/netcord inspection toward accreditation

 


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