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    Scientific Program
The 1st International Congress on
Controversies in Cryopreservation of Stem Cells, Reproductive Cells,
Tissue and Organs (CRYO)
Palacio de Congresos, Valencia, Spain, May 27-30, 2010
  Scientific Program Print

Friday, May 28th, 2010

Plenary Session 1
08:30-10:30 Basic understanding of vitrification
Supported by an unrestricted educational grant from Merck Serono

Chairpersons: A. Arav, Israel, S. Leibo, USA, M. Meseguer, Spain

08:30-08:55  Biosafety of vitrification – the issue of contamination using an open system
                   G. Vajta, Australia

08:55-09:20 Rationale of cryopreservation by Very High Cooling and Warming Rates
                  S. Leibo, USA

09:20-09:45 Chilling injury and vitrification of oocyte
                  A. Arav, Israel

09:45-10:10 Vitrification of bovine and equine embryos - practical considerations
                  G. Seidel, USA (presented by: J. Hasler, USA )

10:10-10:30 Basic physics of small volume vitrification
                  U. Demirci, USA

10:30-11:00 Coffee break

Plenary Session 2
11:00-13:00 Basic understanding of vitrification
Supported by an unrestricted educational grant from Merck Serono

Chairpersons: M. José de los Santos, Spain, N. Kagawa, Japan, P. Nagy, USA
                     G. Vajta, Australia

11:00-12:00 Debate: Cryopreservation of oocytes – slow freezing vs. vitrification
                  L. Parmegiani, Italy
                  P. Nagy, USA

12:00-13:00 Debate: Clinical application – what are the limits?
                  A. Cobo, Spain 
                  N. Noyes, USA

13:00-14:00 Lunch break
Plenary Session 3
14:00-16:00   Animal gametes and embryo freezing
Chairpersons: Y. Barak, Israel, J. Hasler, USA
14:00-14:25    Low - and high - temperature vitrification of sperm as emerging alternatives in
                     preservation of genetic resources
                     I. Katkov, USA

14:25-14:50   Intracelllular ice formation in spermatozoa?
                    G. J. Morris, UK
14:50-15:15   Freeze dried cells for nuclear transfer
                    P. Loi, Italy
15:15-15:40   Embryo freezing in wild animals
                    T. Hildebrandt, Germany
15:40-16:00   What can go wrong during vitrification
                    S. Ledda, Italy
16:00-16:30 Coffee break
Plenary Session 4
16:30-18:10   Cord blood banking, biology, transplantation and tissue regeneration
Chairpersons: A. Nagler, Israel , G. Sanz, Spain

16:30-16:50   Cord Blood Banking – public vs.private network
                    E. Baudoux, Belgium

16:50-17:10   Cord Blood cryobiology: liquid nitrogen & cryoprotectant: for how long can a
                    CBU be preserved?
                    S. Querol, Spain

17:10-17:30   Cord Blood Transplantation: Do we know the best option for alternative donor
                    transplant: MUD vs. CB vs. Haplo?
                    G. Sanz, Spain

17:30-17:50   Cord Blood Transplantations: Strategies to facilitate engraftment, do we know
                    the best one?
                    M. Fernandez, Spain

17:50-18:10   Cord Blood tissue regeneration potential: Mesenchymal stem cells (MSCs)
                    heart, vascular, neurology. Does it exist at all?
                    A. Nagler, Israel
Saturday, May 29th, 2010

Plenary Session 5
08:30-10:30   Stem cell – preservation of future medicine
Supported by an unrestricted educational grant from IBSA / Angelini

Chairpersons: A. Nagler, Israel

08:30-09:00   Adult stem cells vs. embryonic stem cells
                    C. Simon, Spain

09:00-09:30   The use of cord blood stem cells for tissue therapeutics
                    A. Nagler, Israel

09:30-10:00   Stem cells: Are they offering novel strategies for fertility treatments?
                    S. Schlatt, Germany

10:00-10:30   Preclinical and clinical trials of stem cells therapy
                    L. Armstrong, Spain

10:30-11:00 Coffee break

Plenary Session 6
11:00-13:00   Freezing of embryonic & adult stem cells – various techniques

Chairpersons: S. Schlatt, Germany

11:00-11:30   Cryopreservation of human embryonic stem cells
                    F. Holm, Sweden

11:30-11:55   Large animals models in regenerative medicine: the need and the limits
                    F. Gandolfi, Italy

11:55-12:20   Cryopreservation of human Pluripotent stem cells
                    S. Schlatt, Germany

12:20-12:45   Cryopreservation of adult stem cells
                    R. Devireddy, USA
12:45-13:00   Freeze drying of stem cells
                    D. Nathan, Israel
13:00-14:00 Lunch break
Plenary Session 7
14:00-15:30 Fertility, tissue and organ preservation

Chairpersons: P. Patrizio, USA, Z. Shoham, Israel

14:00-14:30    What to freeze: Whole ovary, cortex or other?
                     P. Patrizio, USA

14:30-14:45   In vitro maturation and vitrification of immature oocytes combined with ovarian
                    tissue cryopreservation: A new strategy of fertility preservation
                    G. Fasano, Belgium
14:45-15:15   Duration of function of fresh and frozen ovary transplants
                    DVD Presentation by S. Silber, USA 

15:10-15:30   Where to transplant: Heterotopic or orthotopic sites?
                    M. Sanchez Serrano, Spain

15:30-16:00 Coffee break
Plenary Session 8
16:00-18:00   Embryo freezing
Chairpersons: Liebermann, USA, P. Nagy, USA, J. Van Blerkom, USA
16:00-16:25    What is the best stage for f reezing? From 2PN to blastocyst
                     J. Liebermann, USA
16:25-16:50   Membrane phase behavior and water transport during freezing of mammalian
                    cells studied by FT-IR spectroscopy
                    W. Wolkers, Germany  
16:50-17:15   Embryo freezing: A key to elective single embryo transfer (eSET)
                    F. Mancini, Spain
17:15-17:40   Celluar damage during cryopreservation
                    J. Van Blerkom, USA
17:40-18:00   Freezing animal embryos: domestic, laboratory, wild
                    S. Leibo, USA

Sunday, May 30th, 2010

Plenary Session 9
08:30-10:00    Cryo- bank
Chairpersons: G. Lockwood, UK, G. Stacey, UK

08:30-09:00   Medical and social aspects of cryopreservation of oocytes for fertility
                    G. Lockwood, UK

09:00-09:30   Errors that can occur when applying cryopreservation technology in the real
                    world, and how to minimize these errors
                    J. Hasler, USA
09:30-10:00   Bio-banking of human embryonic stem cell lines: Technical and regulatory 
                    G. Stacey, UK

10:00-10:30 Coffee break

Plenary Session 10
10:30-12:00   Is it possible to restore sperm from cryopreserved tissue or should we
                    always get a fresh one?
Chairpersons: A. Pellicer, Spain, Z. Shoham, Israel

10:30-11:00   Advantages of using cryopreserved TESE against freshly obtained tissue
                    M. Gil-Salom, Spain

11:00-11:30   Sperm quality and freezing
                    Y. Barak, Israel
11:30-12:00   Clinical evaluation of the harmful effects of sperm freezing and its current
                    N. Garrido, Spain

12:00- 12:30  Closing session

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