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The 1st International Congress on
Controversies in Cryopreservation of Stem Cells, Reproductive Cells,
Tissue and Organs (CRYO)
Palacio de Congresos, Valencia, Spain, May 27-30, 2010
 
  Workshops Print

 
Workshop No. 1 - Valencia
HANDS-ON WORKSHOP: OOCYTE AND EMBRYO VITRIFICATION -
THE CRYOTOP METHOD

(Open for a limited number)


The workshop is organized by the IVI group in Valencia, directed by A. Cobo and N. Kagawa.

VITRIFICATION OF OOCYTES WORKSHOP, VALENCIA, SPAIN
Thursday, May 27, 2010, 09:00-18:00


9:00-13:00     THEORETICAL SESSION
Supported by an unrestricted educational grant from Ferring
 
09:00-09:05   Introduction
                    A. Cobo, Spain
 
09:05-09:40   Fundamentals of Cryobiology
                    S. Leibo, USA
 
09:40-10:30   Basic principles of vitrification 
                    A. Arav, Israel
 
10:30-11:00   Coffee break

11:00-11:45   Different approaches of Vitrification
                    G. Vajta, Australia

11:45-12:45   The Cryotop method for oocyte Vitrification. Description of the technique. 
                    Clinical results 
                    N. Kagawa, Japan
 
Live demonstration of using the Cryotop Method vitrifying oocyte with explanation by  Prof. Kagawa
 
Aim of the theoretical session: To introduce the biophysical and biochemical principles involved in cryobiology;
  • Ice formation
  • Slow freezing procedures
  • Seeding
  • Freezing curves
  • The physical principles of Vitrification, introduction to minimum volume methods, cooling rates and different Vitrification devices.
12:45-14:00   Lunch
  
14:00-18:00   HANDS-ON WORKSHOP: OOCYTE AND EMBRYO VITRIFICATION - THE
                    CRYOTOP METHOD:         
                    Director: A. Cobo, Spain
 
The aim of this interactive workshop is to gain the practical use of management skills to carry out the Cryotop Vitrification method providing a unique opportunity for embryologist to learn about and practice an improved method of Vitrification.

In addition to the "hands-on" laboratory program, general principles of cryobiology, minimum volume methods, cooling rates and different Vitrification devices will be discussed in a scheduled program of talks and demos performed by recognized scientists top in the field of cryobiology. Participants are embryologists and researchers working in this particular discipline.

Participants should have the pre-requisite of embryo handling skills.
 
Workshop fee including Congress registration: €2,500 (Two Thousand Five Hundred Euro). 


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