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The 1st International Congress on
Controversies in Cryopreservation of Stem Cells, Reproductive Cells,
Tissue and Organs (CRYO)
Palacio de Congresos, Valencia, Spain, May 27-30, 2010
 
  Workshops Print

Workshop No. 2 - Valencia
HANDS-ON WORKSHOP: OOCYTE AND EMBRYO VITRIFICATION -
THE CRYOTOP METHOD

(Open for a limited number)


The workshop is organized by the IVI group in Valencia and directed by A. Cobo and N. Kagawa.

VITRIFICATION OF OOCYTES WORKSHOP, VALENCIA, SPAIN
Thursday, May 27, 2010, 09:00-18:00


9:00-13:00     THEORETICAL SESSION (Valencia)

09:00-09:05    Introduction
                    A. Cobo, Spain  
 
09:05-09:40   Fundamentals of Cryobiology
                    S. Leibo, USA

09:40-10:30   Basic principles of vitrification 
                    A. Arav, Israel

10:30-11:00   Coffee break
 
11:00-11:45   Different approaches of Vitrification 
                    G. Vajta, Australia
 
11:45-12:45   The Cryotop method for oocyte Vitrification. Description of the technique.
                    Clinical results 
                    N. Kagawa, Japan
 
Live demonstration of using the Cryotop Method vitrifying oocyte with explanation by  Prof. Kagawa
 
Aim of the theoretical session: To introduce the biophysical and biochemical principles involved in cryobiology;
  • Ice formation
  • Slow freezing procedures
  • Seeding
  • Freezing curves
  • The physical principles of Vitrification, introduction to minimum volume methods, cooling rates and different Vitrification devices.
12:45-14:00    Lunch
    
14:00-18:00    HANDS-ON WORKSHOP (Valencia): OOCYTE AND EMBRYO 
                     VITRIFICATION -  THE CRYOTOP METHOD:
                     Director: N. Kagawa, Japan
 
The aim of this interactive workshop is to gain the practical use of management skills to carry out the Cryotop Vitrification method providing a unique opportunity for embryologist to learn about and practice an improved method of Vitrification.

In addition to the "hands-on" laboratory program, general principles of cryobiology, minimum volume methods, cooling rates and different Vitrification devices will be discussed in a scheduled program of talks and demos performed by recognized scientists top in the field of cryobiology. Participants are embryologists and researchers working in this particular discipline.

Applicants should have the pre-requisite of embryo handling skills.

Workshop fee including Congress registration: €2,500 (Two Thousand Five Hundred Euro). 


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