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Workshop No. 2
HANDS-ON WORKSHOP: OOCYTE VITRIFICATION IN
A CLOSED SYSTEM |
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(Open for a limited number) |
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Thursday, April 7, 2011, 09:00-18:00 |
| 09:00-13:00 |
THEORETICAL SESSION |
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Moderators: Ana Cobo, Spain; Masashige Kuwayama, Japan; Amir Arav, Israel |
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| 09:00-09:05 |
Introduction
A. Cobo, Spain
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| 09:05-09:40 |
Fundamentals of Cryobiology
S. Leibo, USA
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| 09:40-10:15 |
Different approaches of Vitrification
G. Vajta, Australia
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| 10:15-10:45 |
Coffee Break
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| 10:45-11:15 |
Vitrification practice made perfect
A. Arav, Israel
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| 11:15-11:45 |
The Cryotop method for oocyte and embryo vitrification?
M. Kuwayama, Japan
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| 11:45-13:00 |
Live demonstration of using the Cryotop Method vitrifying oocyte with explanation by
A. Cobo, Spain and M. Kuwayama, Japan
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Aim of the theoretical session: To introduce the biophysical and biochemical principles involved in cryobiology: |
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Ice formation |
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Slow freezing procedures |
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Seeding |
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Freezing curves |
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The physical principles of Vitrification, introduction to minimum volume methods, cooling rates and different Vitrification devices |
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| 13:00-14:00 |
Lunch Break |
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| 14:00-18:00 |
HANDS-ON WORKSHOP:
OOCYTE VITRIFICATION IN A CLOSED SYSTEM |
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Directors: Amir Arav, Israel; Yona Barak, Israel |
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We will demonstrate the different techniques of closed system Vitrification.
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Each participant will try to vitrify oocytes using Sealed SOPS and the VitMaster. We will review and troubleshoot problems which occur during Vitrification, revealing the methods required to solve these problems.
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Solution effect - are we using a safe solution? |
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Technical skills - how to produce a small drop containing oocytes |
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Open vs Closed system, LN vs. Slush vitrification |
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Using homemade cryo-microscope, explore the crystallization, de-vitrification, fracturing and recrystallization phenomenons |
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Practice Makes Perfect
In addition to the mice oocytes that will be used in the workshop, each participant will have 1000 Glass beads of 100um each (size of oocyte) which will be use for practicing the vitrification procedure.
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Workshop Fee: €2000 (Two thousand euro)
*Workshop & Congress Fee: €2400 (Two thousand four hundred euro)
*Grants the participant access to the IVI Congress and CRYO Congress |
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REGISTRATION IS RUN ON A FIRST COME FIRST SERVE BASIS |
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IVI CONGRESS
Scientifc Program: http://comtecmed.com/ivi/2011/sp.aspx
Registration: http://comtecmed.com/ivi/2011/Registration.aspx |
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