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  Workshop No. 2 Print

 
Workshop No. 2
HANDS-ON WORKSHOP: OOCYTE VITRIFICATION IN
A CLOSED SYSTEM

(Open for a limited number)

Thursday, April 7, 2011, 09:00-18:00

09:00-13:00 THEORETICAL SESSION
Moderators: Ana Cobo, Spain; Masashige Kuwayama, Japan; Amir Arav, Israel
 
09:00-09:05
Introduction
A. Cobo, Spain
 
09:05-09:40
Fundamentals of Cryobiology
S. Leibo, USA
 
09:40-10:15
Different approaches of Vitrification
G. Vajta, Australia
 
10:15-10:45
Coffee Break
 
10:45-11:15
Vitrification practice made perfect
A. Arav, Israel
 
11:15-11:45
The Cryotop method for oocyte and embryo vitrification? This is an introductory lecture to the live demonstration where we will show how to use the open device (by myself exactly the same as last year) and afterwards M.Kuwayama will continue with the closed system. The reason for the adjustment of the time for talks is because we have two demonstrations.
M. Kuwayama, Japan
 
11:45-13:00
Live demonstration of using the Cryotop Method vitrifying oocyte with explanation by
A. Cobo,
Spain and M. Kawayama, Japan

 
Aim of the theoretical session: To introduce the biophysical and biochemical principles involved in cryobiology:
 

Ice formation

Slow freezing procedures

Seeding

Freezing curves

The physical principles of Vitrification, introduction to minimum volume methods, cooling rates and different Vitrification devices
 
13:00-14:00 Lunch Break
 
14:00-18:00
HANDS-ON WORKSHOP:
OOCYTE VITRIFICATION IN A CLOSED SYSTEM
Directors: Amir Arav, Israel; Yona Barak, Israel
 
We will demonstrate the different techniques of closed system Vitrification.
 
Each participant will try to vitrify oocytes using Sealed SOPS and the VitMaster. We will review and troubleshoot problems which occur during Vitrification, revealing the methods required to solve these problems.
 

1)

Solution effect - are we using a safe solution?

2)

Technical skills - how to produce a small drop containing oocytes

3)

Open vs Closed system, LN vs. Slush vitrification

4)

Using homemade cryo-microscope, explore the crystallization, de-vitrification, fracturing and recrystallization phenomenons
 
Practice Makes Perfect
In addition to the mice oocytes that will be used in the workshop, each participant will have 1000 Glass beads of 100um each (size of oocyte) which will be use for practicing the vitrification procedure.
  
Workshop Fee: €2000 (Two thousand euro)
 
*Workshop & Congress Fee
: €2400 (Two thousand four hundred euro)
*Grants the participant access to the IVI Congress and CRYO Congress
 
REGISTRATION IS RUN ON A FIRST COME FIRST SERVE BASIS
 
IVI CONGRESS
Scientifc Program:
http://comtecmed.com/ivi/2011/sp.aspx
Registration: http://comtecmed.com/ivi/2011/Registration.aspx


 


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